Real time identification of biliary anatomy by fluorescence using fluorescein sodium

Abstract

Objective: To develop an experimental animal model technique using sodium fluorescein intraoperative cholangiography. To Determine the dose of sodium
fluorescein necessary for the best display of the gallbladder and biliary tree. Methods: Experimental study. 6 New Zealand white albino rabbits divided into 3
groups depending on the dose of sodium fluorescein (5 mg / kg, 7.5 mg / kg, 15 mg / kg) were used. Prior anesthetic protocol corresponding fluorescein sodium
was administered according included Animal group intravenously. Upper midline incision was used, exteriorization of the liver allow easy identification of extrahepatic
biliary system and subsequently illuminated with fluorescence. Parameters were recorded for each dose. No euthanasia was performed. Environment:
Private Veterinary Centre “Colmillos y Garras”. Results: In all cases, we recorded fluorescence. The dose that allowed the best visualization was 7.5 mg / kg. The average
time in which fluorescence began to be visible in the gallbladder was 5 minutes, the average time in which the total fluorescence was reached was 18 minutes in all cases fluorescence persisted until 30 minutes. Conclusion: Laparoscopic cholecystectomy guied using sodium fluorescein fluorescence and UV light source of 5 mm diameter is feasible. Better visualization is obtained with the dose 7.5 mg / kg.